Atlético, AZ Alkmaar, Dortmund, Dynamo Kyiv, Genk, Midtjylland, Sevilla and Žilina have won their play-off ties to secure places in the UEFA Youth League round of 16.
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Dynamo Kyiv completed the UEFA Youth League round of 16 line-up with a penalty shoot-out triumph over Deportivo La Coruña following a lively encounter in Spain.
After a goalless 90 minutes in Croatia, Atlético also won from the spot, seeing off Hadjuk Split. In Wednesday's earlier play-off matches, Midtjylland rallied from 2-0 down to beat Club Brugge 3-2 in Denmark, whilst Genk recorded an impressive 5-1 victory over Chelsea to go through.
On Tuesday, Dutch side AZ Alkmaar edged a thriller to get the play-offs under way, winning 4-3 on penalties after coming back from 3-1 down against Villarreal with 14 minutes to go. Sevilla took a more direct route with a 1-0 win against Rangers, though they were forced to close the game out with only ten men, and Žilina then beat Inter 2-1 to become the first team from Slovakia to reach the round of 16. Dortmund booked their progress courtesy of a 5-3 defeat of Empoli.
Through to round of 16
Group winners: Benfica, Juventus, Liverpool, Manchester United, Paris Saint-Germain, Real Madrid (holders), Salzburg, Sporting CP
Play-off winners: Atlético, AZ Alkmaar, Dortmund, Dynamo Kyiv, Genk, Midtjylland, Sevilla, Žilina
The UEFA Youth League play-off winners join the teams that finished top of the UEFA Champions League path groups in the round of 16.
In the play-offs, the winners of the eight domestic champions path second-round ties are at home to the runners-up from the UEFA Champions League path group stage.
• Atlético made it to this round for the fifth time, having never failed to get past the group stage in any of this competition's eight editions.
• AZ, Deportivo and Hajduk are on debut while Genk, Villarreal and Žilina made it to the play-offs for the first time.
• Two-time champions Chelsea reached the 2019 final from the play-offs (having entered in the domestic champions path).
Knockout draw (round of 16 onwards): 14 February, Nyon
Round of 16: 1/2 March
Quarter-finals: 15/16 March
Semi-finals: 22 April, Nyon
Final: 25 April, Nyon