Debutants Chelsea and Atlético Madrid face two-time winners Wolfsburg and Lyon respectively, while holders FFC Frankfurt take on LSK Kvinner after the round of 16 draw was made.
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Debutants Chelsea and Atlético Madrid have both been drawn against two-time UEFA Women's Champions League winners, while holders FFC Frankfurt take on Norway's LSK Kvinner after the round of 16 draw was made in Nyon.
The draw (ties 11/12 & 18/19 November)
FC Twente (NED) v FC Barcelona (ESP)
Brescia (ITA) v Fortuna Hjørring (DEN)
Atlético Madrid (ESP) v Olympique Lyonnais (FRA)
Slavia Praha (CZE) v Zvezda-2005 (RUS)
Chelsea (ENG) v Wolfsburg (GER)
KIF Örebro (SWE) v Paris Saint-Germain (FRA)
Verona (ITA) v Rosengård (SWE)
LSK Kvinner (NOR) v FFC Frankfurt (GER, holders)
• Rosengård knocked out Verona 3-0 on aggregate at this stage in 2012/13.
• Fortuna have won all three of their previous ties against Italian opposition.
• As well as past winners Frankfurt (2002, 2006, 2008, 2015), Lyon (2011, 2012) and Wolfsburg (2013, 2014), previous finalists involved are Paris (2015), Zvezda (2009) and Fortuna (2003).
• All three debutants in the round of 32, Atlético, Chelsea and Örebro, went through.
• Twente, who became the first team to beat a German side in the round of 32 when they eliminated Bayern München, are the only remaining club that competed in the qualifying round.
Round of 16: 11/12 November & 18/19 November
Quarter-final & Semi-final draw: 27 November
Quarter-finals: 23/24 & 30/31 March
Semi-finals: 23/24 & 30 April/1 May
Final (Reggio Emilia): 26 May
Emma Hayes, Chelsea manager
Lindsay Horan, Paris player
Ada Hegerberg, Lyon player
Sydney Payne, Fortuna player
Olivia Schough, Sweden & Eskilstuna United (in next season's competition)